Journal: iScience

Article Title: Nucleus-forming jumbophage PhiKZ therapeutically outcompetes non-nucleus-forming jumbophage Callisto

doi: 10.1016/j.isci.2024.109790

Figure Lengend Snippet: Bactericidal spectrum and efficiency of plating (EOP) of phage Callisto

Article Snippet: P. fluorescens ATCC13525 , American Type Culture Collection , –.

Techniques:

Journal: iScience

Article Title: Nucleus-forming jumbophage PhiKZ therapeutically outcompetes non-nucleus-forming jumbophage Callisto

doi: 10.1016/j.isci.2024.109790

Figure Lengend Snippet:

Article Snippet: P. fluorescens ATCC13525 , American Type Culture Collection , –.

Techniques: Virus, Recombinant, Electron Microscopy, Cloning, Plasmid Preparation, Software

Journal:

Article Title: Degradation of Triphenyltin by a Fluorescent Pseudomonad

doi:

Figure Lengend Snippet: Biodegradation of TPT by ATCC strains of fluorescent pseudomonads in SG medium supplemented with TPT. The concentrations of DPT produced by TPT (130 μM) degradation were determined by post-column HPLC analysis. Similar results were obtained in three independent experiments. Symbols: ●, P. chlororaphis ATCC 9446; ▴, P. fluorescens ATCC 13525; ⧫, P. putida ATCC 12633; ■, P. aeruginosa ATCC 15692.

Article Snippet: However, it should be noted that the pyoverdines produced by P. aeruginosa ATCC 15692, P. fluorescens ATCC 13525, and P. chlororaphis ATCC 9446 that exhibit TPT-DF activity have a closely related structure and cross-reactivity to the iron transport system, whereas P. putida ATCC 12633 produces a strain-specific pyoverdine ( 10 , 18 , 27 ).

Techniques: Produced

Journal:

Article Title: Degradation of Triphenyltin by a Fluorescent Pseudomonad

doi:

Figure Lengend Snippet: Biodegradation of TPT by ATCC strains of fluorescent pseudomonads in SG medium supplemented with TPT. The concentrations of DPT produced by TPT (130 μM) degradation were determined by post-column HPLC analysis. Similar results were obtained in three independent experiments. Symbols: ●, P. chlororaphis ATCC 9446; ▴, P. fluorescens ATCC 13525; ⧫, P. putida ATCC 12633; ■, P. aeruginosa ATCC 15692.

Article Snippet: P. chlororaphis CNR15, which was isolated in this study, P. chlororaphis ATCC 9446, Pseudomonas fluorescens ATCC 13525, P. putida ATCC 12633, and Pseudomonas aeruginosa ATCC 15692 were grown on succinate-glycerol (SG) medium made up of 1.0 g of K 2 HPO 4 , 1.0 g of KH 2 PO 4 , 1.0 g of (NH 4 ) 2 SO 4 , 0.4 g of MgCl 2 , 0.5 g of yeast extract, 4.0 g of succinate, and 1.0 ml of glycerol per liter and adjusted to pH 6.8 by adding the required volume of 2 N NaOH prior to sterilization.

Techniques: Produced

Journal:

Article Title: Bacterial Species Determination from DNA-DNA Hybridization by Using Genome Fragments and DNA Microarrays

doi: 10.1128/AEM.67.8.3677-3682.2001

Figure Lengend Snippet: Similarity dendrogram (UPGMA) of hybridization profiles of 338 genome fragments spotted on the microarray. Clusters F (98.7%), C (94.1%), A (91.8%), P (100%), and Y (100%) comprise genome fragments from the reference strains P. fluorescens ATCC 13525T, P. chlororaphis ATCC 9447, P. aeruginosa ATCC 15692, and P. putida ATCC 12633T. Clusters V to Z comprise genome fragment from different reference strains, except cluster Y.

Article Snippet: In contrast, genomic fragments with a large angle (low evenness) tend to show a low average signal ratio with high standard deviation, indicating that they showed appreciable hybridization signal only to the closely related strains and hence are considered variable sequences. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 6 caption a7 (a) Evenness value (θ E ) scatter diagram, with average and SD of log hybridization signal ratio. (b) θ E values by genome fragment, ID 1 to 92, 93 to 182, 183 to 278, and 279 to 338 originated from P. fluorescens ATCC 13525 T , P. chlororaphis ATCC 9447, P. putida ATCC 12633 T , and P. aeruginosa ATCC 15692, respectively.

Techniques: Hybridization, Microarray

Journal:

Article Title: Bacterial Species Determination from DNA-DNA Hybridization by Using Genome Fragments and DNA Microarrays

doi: 10.1128/AEM.67.8.3677-3682.2001

Figure Lengend Snippet: (a) Evenness value (θE) scatter diagram, with average and SD of log hybridization signal ratio. (b) θE values by genome fragment, ID 1 to 92, 93 to 182, 183 to 278, and 279 to 338 originated from P. fluorescens ATCC 13525T, P. chlororaphis ATCC 9447, P. putida ATCC 12633T, and P. aeruginosa ATCC 15692, respectively. The solid line and dotted lines (horizontal) indicate average and SD, respectively.

Article Snippet: In contrast, genomic fragments with a large angle (low evenness) tend to show a low average signal ratio with high standard deviation, indicating that they showed appreciable hybridization signal only to the closely related strains and hence are considered variable sequences. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 6 caption a7 (a) Evenness value (θ E ) scatter diagram, with average and SD of log hybridization signal ratio. (b) θ E values by genome fragment, ID 1 to 92, 93 to 182, 183 to 278, and 279 to 338 originated from P. fluorescens ATCC 13525 T , P. chlororaphis ATCC 9447, P. putida ATCC 12633 T , and P. aeruginosa ATCC 15692, respectively.

Techniques: Hybridization

Journal:

Article Title: Application of Siderotyping for Characterization of Pseudomonas tolaasii and " Pseudomonas reactans " Isolates Associated with Brown Blotch Disease of Cultivated Mushrooms

doi:

Figure Lengend Snippet: Heterologous PVD-mediated 59Fe incorporation by “P. reactans” strains belonging to the eight siderovars. Ordinate values correspond to 59Fe radioactivity expressed in counts per minute as measured after 20 min of incubation (see Materials and Methods). Abscissa numbers 1 to 36 correspond to the structurally different PVDs tested, originating from the following bacterial strains (PVD numbers are given in parentheses): Pseudomonas strain E8 (1), P. syringae ATCC 19310 (2), P. fluorescens 9AW (3), P. putida ATCC 12633 (4), P. fluorescens 51W (5), P. aeruginosa Pa6 (6), P. fluorescens CCM 2798 (7), P. fluorescens CHA0 (8), P. tolaasii NCPPB 2192 (9), P. aeruginosa ATCC 27853 (10), P. fluorescens ii (11), P. fluorescens SB8.3 (12), P. fluorescens ATCC 17400 (13), P. fluorescens 1.3 (14), Pseudomonas strain 267 (15), P. fluorescens ATCC 13525 (16), P. aeruginosa ATCC 15692 (17), P. fluorescens strain 18.1 (18), P. fluorescens 12 (19), P. fluorescens CFBP 2392 (20), Pseudomonas strain L1 (21), Pseudomonas sp. strain ATCC 15915 (22), P. putida WCS358 (23), P. monteilii CFML 90-54 (24), “P. mosselii” CFML 90-77 (25), P. rhodesiae CFML 92-104 (26), P. veronii CFML 92-124 (27), Pseudomonas sp. strain CFML 90-33 (28), Pseudomonas sp. strain CFML 90-51 (29), Pseudomonas sp. strain CFML 90-52 (30), Pseudomonas sp. strain CFML 95-307 (31), Pseudomonas sp. strain 2908 (32), Pseudomonas sp. strain A214 (33), P. fluorescens PL7 (34), P. fluorescens PL8 (35), and the PVD synthesized by the strain under investigation (36). The counts per minute were corrected for the blank values obtained in assays without bacteria.

Article Snippet: P. aeruginosa ATCC 15692, P. fluorescens ATCC 13525, and P. fluorescens ATCC 17400 were from the American Type Culture Collection, Manassas, Va. Pseudomonas monteilii CFML 90.54 ( 12 ) and other strains of the Collection de la Faculté de Médecine de Lille (CFML), P. fluorescens 51W ( 25 ), P. fluorescens 12 ( 15 ), and P. fluorescens 18.1 ( 6 ) were kindly provided by D. Izard (Université de Lille 2, Lille, France), S. Shivaji (Center of Cellular and Molecular Biology, Hyderabad, India), H. Budzikiewicz (Universität zu Köln, Cologne, Germany), and M. Champomier-Verges (INRA, Jouy-en-Josas, France), respectively. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Open in a separate window Open in a separate window a LMG Collection, Laboratory of Microbiology, Ghent, Belgium. b The PVD-deficient strain PS7.5 was grouped in P. tolaasii sv.

Techniques: Radioactivity, Incubation, Synthesized

Journal:

Article Title: Application of Siderotyping for Characterization of Pseudomonas tolaasii and " Pseudomonas reactans " Isolates Associated with Brown Blotch Disease of Cultivated Mushrooms

doi:

Figure Lengend Snippet: Heterologous PVD-mediated iron incorporation in “ P. reactans ” isolates and some reference strains

Article Snippet: P. aeruginosa ATCC 15692, P. fluorescens ATCC 13525, and P. fluorescens ATCC 17400 were from the American Type Culture Collection, Manassas, Va. Pseudomonas monteilii CFML 90.54 ( 12 ) and other strains of the Collection de la Faculté de Médecine de Lille (CFML), P. fluorescens 51W ( 25 ), P. fluorescens 12 ( 15 ), and P. fluorescens 18.1 ( 6 ) were kindly provided by D. Izard (Université de Lille 2, Lille, France), S. Shivaji (Center of Cellular and Molecular Biology, Hyderabad, India), H. Budzikiewicz (Universität zu Köln, Cologne, Germany), and M. Champomier-Verges (INRA, Jouy-en-Josas, France), respectively. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Open in a separate window Open in a separate window a LMG Collection, Laboratory of Microbiology, Ghent, Belgium. b The PVD-deficient strain PS7.5 was grouped in P. tolaasii sv.

Techniques:

Journal:

Article Title: Application of Siderotyping for Characterization of Pseudomonas tolaasii and " Pseudomonas reactans " Isolates Associated with Brown Blotch Disease of Cultivated Mushrooms

doi:

Figure Lengend Snippet: IEF profiles of PVDs for some “P. reactans” isolates and some other pseudomonads having similar uptake efficiencies. PVDs originated from the indicated strains: lane 1, “P. reactans” PL24.1 (sv. 1); lane 2, P. fluorescens ATCC 17400; lane 3, “P. reactans” PL10.9 (sv. 6); lane 4, P. fluorescens 51W; lane 5, P. monteilii CFML 90.54; lane 6, “P. reactans” PL4.14 (sv. 7); lane 7, P. fluorescens 12; lane 8, P. fluorescens 18.1; lane 9, P. aeruginosa ATCC 15692; lane 10, “P. reactans” PS11.4 (sv. 2); lane 11, “P. reactans” PS3b (sv. 5); lane 12, P. rhodesiae CFML 92.104; lane 13, P. veronii CFML 92.124; lane 14, “P. reactans” PS7.2 (sv. 3); lane 15, P. fluorescens ATCC 13525.

Article Snippet: P. aeruginosa ATCC 15692, P. fluorescens ATCC 13525, and P. fluorescens ATCC 17400 were from the American Type Culture Collection, Manassas, Va. Pseudomonas monteilii CFML 90.54 ( 12 ) and other strains of the Collection de la Faculté de Médecine de Lille (CFML), P. fluorescens 51W ( 25 ), P. fluorescens 12 ( 15 ), and P. fluorescens 18.1 ( 6 ) were kindly provided by D. Izard (Université de Lille 2, Lille, France), S. Shivaji (Center of Cellular and Molecular Biology, Hyderabad, India), H. Budzikiewicz (Universität zu Köln, Cologne, Germany), and M. Champomier-Verges (INRA, Jouy-en-Josas, France), respectively. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Open in a separate window Open in a separate window a LMG Collection, Laboratory of Microbiology, Ghent, Belgium. b The PVD-deficient strain PS7.5 was grouped in P. tolaasii sv.

Techniques: